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In Vitro Assays Development & Good In Vitro Method Practices

This training will illustrate various aspects of the OECD Good In Vitro Methods Practices (GIVIMP) guidance document (STA 286) using assays/techniques/test systems that have been developed recently by the different trainers. It aims to provide the participants with hands-on, practical skills in how to apply GIVIMP in their daily work in the laboratory, and at the same time learn about modern techniques used for in vitro testing.

Among trainers besides RISE (a polytechnic research institute and member of EU-NETVAL), there will be acCELLerate (a specialist for Assay Ready Cells and Cell Banking), Evercyte and SenzaGen.

Participants will learn among other things:

  • Evaluate robustness of test systems and quality control procedures (AcCELLerate and Evercyte)
  • Choice of test, reference, and control items; solubility verification; development of acceptance criteria (SenzaGen)
  • Method performance and SOP development (RISE)

The training will start with 0.5 day lectures and will be followed by 1.5 days of practical training (Hands on-training) where participants, divided in small groups will get the chance to manipulate and see the critical steps of the methods with the experts. This is a perfect way to quickly approach a method, as well as data analysis and interpretation.

Draft Agenda will be available soon or contact us nathalie.belot@altertox.be

Registration fee includes the participant booklet, catering and social dinner.

In Vitro Lungs Model

This two days hands-on training hosted by Epithelix will cover practical use of respiratory in vitro 3D tissues and exposure devices to evaluate acute and repeated dose inhalation toxicity. To mimic systemic context, interconnection strategies of lung tissues will be presented.

The training will only take place if minimum of 8 participants is reached.

Registration fee includes the participant booklet, catering and social dinner.

The training will start with 0.5 day lectures and will be followed by 1.5 days of practical training (Hands on-training) where participants, divided in small groups will get the chance to manipulate and see the critical steps of the methods with the experts. This is a perfect way to quickly approach a method, as well as data analysis and interpretation.

Registration fee includes the participant booklet, catering and social dinner.

Acute and chronic cardiotoxicity

The course is a two-day laboratory workshop in Leiden. This training instructs the attendee in best practices procedures for in silico and in vitro assays related to acute and chronic cardiotoxicity.

Participants will learn among other things:

  • Regulatory context to apply these methodologies as well as strengths, weaknesses and future prospects (UCB – Dr Delaunois)
  • Cell handling of human iPSC-derived cardiomyocytes (e.g. thawing procedure, cells seeding) (NCardia)
  • Preparation of cryopreserved hiPSC-derived cardiomyocytes in 48/96 or 348 well plates (NCardia)
  • Exposure of cells with cardiotoxic compounds using different readouts such as CiPA-like MEA (Micro-electrode array) and calcium flux assay (NCardia)
  • Risk Assessment, validation, analysis interpretation and decision making using hiPS-cells for early drug discovery and development (Jannsen Pharma – Dr. Kreir)
  • In silico assessment of the effects of various compounds in MEA/hiPSC-CM assays: modelling and numerical simulations (inria)

The training will start with half day lectures and will be followed by 1.5 days of practical training (Hands on-training) where participants, divided in small group will get the chance to manipulate and see the critical steps of the methods with the experts. This is a perfect way to quickly approach a method, as well as data analysis and interpretation.

Draft Agenda will be soon available.

The training will only take place if a minimum of 4 participants is reached.

Registration fee includes the participant booklet, catering and social dinner.

Irritation oculaire in vitro

  • Fee-based
  • 21 novembre 2019
  • Lyon, France
    • Lors de cette formation, le contexte général de l’évaluation toxicologique sera abordé ainsi  que le statut   actuel   de   la   validation   des   méthodes alternatives en Europe (Règlement   CE   1223/2009, SCCS)   ainsi   que   les   recommandations   internationales (OECD Test Guidelines, ICCR).
    • Après   introduction   des   tests   et   méthodes  in vitro validés   pour   les   cosmétiques,   les avantages  et   les   limites   de   chaque méthode d’essai   seront appréhendées   par   des   cas  concrets.   Les   participants   auront   l’opportunité   de   traiter des données et de les interpréter.  Des méthodes non réglementaires seront également abordées (irritation oculaire faible). 

    La formation aura lieu s’il y a au moins 5 participants inscrits.

Skin Sensitization

At this two-days training, experts will put emphasis on sensitization models and skin sensitization quantitative risk assessment. Introduction and application of validated methods (DPRA, LuSens), a coculture model (COCAT), in silico integrating tools and their application in risk assessment will be demonstrated. The training will focus on data assessment, testing strategies and their use in skin sensitization quantitative risk assessment.

Among future trainers besides Trier University, there will be BASF, Coty (to be confirmed), Edelweiss Connect.

The participants will learn among other things:

  • Alternatives to animal testing to assess sensitization potential and potency (Dr B. Blömeke – Trier University)
  • Introduction to skin sensitization validated methods (OECD TG 442C, TG 442D and TG 442E) (BASF)
  • COCAT – A HaCaT/THP-1 coculture model to estimate skin sensitization potential and potency in vitro (Trier University)
  • Quantitative risk assessment-case study (Coty)

The training will start with half day lectures and will be followed by 1.5 days of practical training (Hands on-training) where participants, divided in small groups will get the chance to manipulate and see the critical steps of the methods with the experts. This is a perfect way to quickly approach a method, as well as data analysis and interpretation.

Draft Agenda will be soon available.

Registration fee includes the participant booklet, catering and social dinner.

Skin and Eye Irritation

  • Fee-based
  • 3-4 december 2019
  • Brussels, Belgium

Irritation oculaire in vitro

  • Fee-based
  • 19 Mars 2020
  • Lyon, France
  • Lors de cette formation, le contexte général de l’évaluation toxicologique sera abordé ainsi  que le statut   actuel   de   la   validation   des   méthodes alternatives en Europe (Règlement   CE   1223/2009, SCCS)   ainsi   que   les   recommandations   internationales (OECD Test Guidelines, ICCR).
  • Après   introduction   des   tests   et   méthodes  in vitro validés   pour   les   cosmétiques,   les avantages  et   les   limites   de   chaque méthode d’essai   seront appréhendées   par   des   cas  concrets.   Les   participants   auront   l’opportunité   de   traiter des données et de les interpréter.  Des méthodes non réglementaires seront également abordées (irritation oculaire faible). 

La formation aura lieu s’il y a au moins 5 participants inscrits.

contact us for more info

+32 (0)485 52 39 45